Layer by Layer RNA encapsulation for genetic therapy nanodevices construction D.Sanz
نویسندگان
چکیده
The Layer by Layer (LBL) technique, based on the alternative assembly of oppositely charged polyelectrolyte molecules, is a very versatile tool for the fabrication of thin polymer film with controlled features at the nanoscale [2]. We have applied the LBL technique to encapsulate purified total RNA between polyelectrolyte layers as a negative charged biopolyelectrolyte in Chitosane/Alginate/RNA and PAA/PEI/RNA systems on planar surfaces and colloidal PLGA particles.
منابع مشابه
Thermal vibration analysis of double-layer graphene embedded in elastic medium based on nonlocal continuum mechanics
This paper presents the thermal vibration analysis of double-layer graphene sheet embedded in polymer elastic medium, using the plate theory and nonlocal continuum mechanics for small scale effects. The graphene is modeled based on continuum plate theory and the axial stress caused by the thermal effects is also considered. Nonlocal governing equations of motion for this double-layer graphene s...
متن کاملThermal vibration analysis of double-layer graphene embedded in elastic medium based on nonlocal continuum mechanics
This paper presents the thermal vibration analysis of double-layer graphene sheet embedded in polymer elastic medium, using the plate theory and nonlocal continuum mechanics for small scale effects. The graphene is modeled based on continuum plate theory and the axial stress caused by the thermal effects is also considered. Nonlocal governing equations of motion for this double-layer graphene s...
متن کاملA Novel Microfluidic Droplet Manipulation Method for Fabrication of Reverse-phase Two Layer Layer-by-layer Protein Microcapsules
This paper presents a novel droplet manipulation approach using high flow resistance microstructures for transferring microdroplets through different reagent streams for generating 2 layered polymeric-shell PSS/PEI microcapsules for biomolecular encapsulation. Our approach demonstrates the formation of protein capsules using the principle of ReversePhase Layer-by-Layer (RP-LbL) polyelectrolyte ...
متن کاملGDL construction effects on distribution of reactants and electrical current density in PEMFC
In this article, a two dimensional pore scale model of polymeric fuel cell, which is promising of a clean and renewable energy production, is presented here. Let reactive gases behave as an ideal gas; inhomogeneous anisotropic structure of the gas diffusion layer, is contemplated as a random generated circular porous media. Lattice Boltzmann method is applied to inquire the fluid flow and mass ...
متن کامل“Self‐Peel‐Off” Transfer Produces Ultrathin Polyvinylidene‐Fluoride‐Based Flexible Nanodevices
Here, a new strategy, self-peel-off transfer, for the preparation of ultrathin flexible nanodevices made from polyvinylidene-fluoride (PVDF) is reported. In this process, a functional pattern of nanoparticles is transferred via peeling from a temporary substrate to the final PVDF film. This peeling process takes advantage of the differences in the work of adhesion between the various layers (th...
متن کامل